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KMID : 0545120110210030284
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 3 p.284 ~ p.292
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Isolation and Characterization of Endocellulase-Free Multienzyme Complex from Newly Isolated Thermoanaerobacterium thermosaccharolyticum Strain NOI-1
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Suphavadee Chimtong
Chakrit Tachaapaikoon
Patthra Pason
Khin Lay Kyu
Akihiko Kosugi
Yutaka Mori
Khanok Ratanakhanokchai
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Abstract
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An endocellulase-free multienzyme complex was produced by a thermophilic anaerobic bacterium, Thermoanaerobacterium thermosaccharolyticum strain NOI-1, when grown on xylan. The temperature and pH optima for growth were 60oC and 6.0, respectively. The bacterial cells were found to adhere to insoluble xylan and Avicel. A scanning electron microscopy analysis showed the adhesion of xylan to the cells. An endocellulase-free multienzyme complex was isolated from the crude enzyme of strain NOI-1 by affinity purification on cellulose and Sephacryl S-300 gel filtration. The molecular mass of the multienzyme complex was estimated to be about 1,200 kDa. The multienzyme complex showed one protein on native PAGE, one xylanase on a native zymogram, 21 proteins on SDS-PAGE, and 5 xylanases on a SDS zymogram. The multienzyme complex consisted of xylanase, β-xylosidase, α-L-arabinofuranosidase, β-glucosidase, and cellobiohydrolase. The multienzyme complex was effective in hydrolyzing xylan and corn hulls. This is the first report of an endocellulase-free multienzyme complex produced by a thermophilic anaerobic bacterium, T. thermosaccharolyticum strain NOI-1.
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KEYWORD
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Endo-cellulase-free multienzyme complex, Thermoanaerobacterium thermosaccharolyticum, thermophilic anaerobic bacterium, xylanase
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